Enhancement of the Efficacy of Photodynamic Therapy against Uropathogenic Gram-Negative Bacteria Species

Antimicrobial photodynamic therapy (aPDT) was demonstrated to be effective against various species of Gram-positive bacteria. However, the complex structure a Gram-negative bacteria envelope limits application aPDT. Thus, goal this study improve efficiency antimicrobial with Fotoditazin uropathogenic The non-ionic detergent Triton X-100 and emulsifier Tween 80 were tested. effect extracellular photosensitizer on aPDT efficacy analyzed. Moreover, irradiation regime optimized in terms output power emitting mode. It found that at 10% vol enhanced E. coli up 52%. subsequent observation that, when removed from space, decreased dramatically. As for mode, an increase laser led efficacy. pulsed mode did not affect order achieve optimal efficacy, should irradiated 450-mW presence environment. it noted K. pneumoniae significantly lower than other species. developed technique may native environment microorganisms.